Non-Surgical Sterilization

Welcome to the Spay FIRST non-surgical sterilization page. This page is here to provide information on products and compounds that make it possible to prevent litters when traditional spay or neutering surgeries are out of reach due to poverty, distance and other challenges.   Watch as this page grows!

Nurses assist with non-surgical sterilization

Surgical spaying and neutering is safe and permanent; this page is not intended to highlight alternatives to standard veterinary procedures where these procedures are affordable and can be provided for the majority of dogs and cats.

This site is intended to provide information about options for the millions of unwanted animals for whom surgical sterilization is out of range due to a lack of funding, clinics, and human resources (surgeons) and for whom an unwanted litter can mean starvation and death for mom and her litter. Not a single continent is untouched by this problem.

Dr H and  Vanessa assist with non-surgical dog sterilization

Estimates vary widely, but at least 400 million dogs and roughly the same number of cats exist worldwide and around 300 million (three quarters) of each are unwanted. Most live in nations in which animal welfare laws do not exist or cannot be enforced; many are chased away or beaten for simply begging for a scrap of food. Non-surgical options will prevent untold suffering for just pennies each.

The average life of a street dog is roughly three years. Non-surgical sterilization is the only way to develop humane population control programs in impoverished places and to do so in a big enough way to make a difference.

SpayFirst performs non-surgical sterilization and neutering on cats and dogs Additionally, tens of thousands of people in developing nations die of rabies each year and over 98 percent of cases come from dog bites. Street dogs are not seen as a friend in need; they are despised as a dangerous vector; many become targets of cruel killing campaigns.   Stopping new generations of street dogs from being born is vital to wiping out rabies while placing animal welfare into the forefront.

Calcium chloride: a video for veterinarians.  This video shows the use of compounded calcium chloride dihydrate for non-surgical castration of male dogs.  Use of compounded drugs must be carried out in accordance with state or province regulations and with a valid Veterinary Client Patient Relationship (VCPR) in place.  Technique modified from earlier research by Kuladip Jana, Prabhat K Samanta and Raffaella Leoci.


Non-surgical sterilization can stop the suffering. We can turn fear into compassion and make a lasting difference for animals and the communities they live in.

To join us in this effort…

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Help save unwanted cats and dogs - non-surgical spaying and neutering helps prevent unwanted pets and animals

  • $65 pays for calcium chloride neutering for over 20 male dogs
  • $200 provides calcium chloride neutering, a rabies vaccine and dewormer for 50 male dogs on a tribal program
  • $250 provides gas money to get a veterinarian to a reservation in the northern Midwest
  • $1250 provides a full clinic day that includes 40 spays for female dogs or cats, and 50 calcium chloride neuters for male dogs and cats, along with rabies vaccines and deworming



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  • Is this safe and available in the USA? If so, please provide where and which orgs are now using this method.

    • Ruth Steinberger

      Yes. it may be ordered from or is available as a compounded drug and sold only to veterinarians. There are precautions and like all drugs or surgery there are risks. Thank you!

  • RaymundoFries3

    Good post , Apropos , if someone needs to fill out a a form , my family saw a fillable document here

  • Jessica

    Very good non classical way. I would love you to tell me, please, whether this way is sold around the world or if it is only available in the Usa. I do admire people who participate in this regards. Many animals around the world do need this to avoid overpopulation. If it is not sold around the world, I do kindly suggest to make the right effort to do so. Because, I am sure that many people do want to use this new method to avoid overpopulation. I would also know the price of it. Thank you very much for this and for your reply to my questions.

    • Niobe

      This non surgical neutering technique is not patented and the ingredients to make it, calcium chloride, is availible worldwide. There is nothing standing in the way except knowledge of this very simple and cheap neutering solution.

  • Lesley

    Has Spay First been using CaCL for castrations and monitoring the animals afterwards? I just participated in a clinic in Punta Cana where it was used and there were complications in too many animals. 100 dogs were castrated using CaCL and 12 dogs that we know of ended up with swollen, raw testicles requiring an antibiotic spay and meds and they are being monitored. These are dogs who all had owners. There were many street dogs who also received CaCL and we have no idea how many had the same reaction – the local rescue group is trying to walk the streets and check on them but they could be curled up somewhere in agony. The group I volunteer with has said they will no longer participate in this type of neutering until there is much further research and the risks and complications are reduced. Has anyone else had similar experience?

    • Jodi

      Were the 12 dogs that ended up with the reaction injected by the same individual(s)? From the research I’ve done, the injection itself requires finesse to alleviate pressure and ultimately an adverse reaction. Has no one replied to your inquiry?

    • Ruth Steinberger

      As the founder of Spay FIRST, and with significant experience with veterinarians who have used this, my comment is that it is technique dependent and like most things in medicine and surgery, there is no reason to expect not to have problems if someone is untrained. My understanding is that at Punta Cana the training set up that was to be available was not there, no place existed for the video loop to be run for the veterinarians and the training was basically able to be bypassed. Ultimately, no one knew who did which dog, and there were some significant mistakes made (for example one dog was reportedly injected twice in the same testicle).

      Surgical spay/neuter is the gold standard and probably will be for a while, but the fact is that we will not reach most of the animals suffering in chronic poverty without non-surgical, low-cost products that are effective and easy to administer. Globally, in impoverished areas, most dogs are male because people do not enable the females to survive. Calcium chloride makes it possible to sterilize male dogs for a minimal amount of money and time, leaving greater resources for females. It is an important tool. The clinic at Punta Cana is mainly a statement about organization and protocol, not a statement about calcium chloride.

      • Jenny Kirchner

        thanks Ruth, training and skill is typically an issue in the third world. in sayin that 4000 dogs and cats are killed every year at the SPCA in Auckland NZ alone from lack of homes. anything that can alleviate these numbers is a step in the right direction. i am wondering if this a lack of empathy and responsibility of people in general and lack of access is a problem. certainly is in fiji from what i have seen over the last 10 years. all my pets since i was a kid were desexed as i can not possibly look after all the offspring of one female cat or dog. agreed it is so sad for the female dogs that do survive, 1 female to every 10 male, what an awful time they have. thank you for doing this, a few billion other people do nothing, thats something.

    • Jenny Kirchner

      thanks Lesley, its such a big issue, at least this is something. the dogs suffer anyways, they would die from starvation/accidents or illness regardless. im in fiji and the dogs get poisoned by local council, its horrific. i paid for an exceptional vets flight (she donated her time)from nz to come and with a small volunteer team and desexed 44 cats and dogs in 5 days. it was recognised as just a drop in the ocean for all the hard work. hence this is why i am looking for an alternative and appreciate your experience. thank you

    • Lulu

      Hi Lesley, what is the name of the clinic in Punta Cana? I am hoping to launch something similar here in Argentina and any information from others who have done similar projects would be very useful, thank you!!

  • Parnasudha Karmodak Chakrabort

    Is the method applicable for cats too? And is it safe to apply the method by an untrained Doctor ?

    • Kit

      are you insane lady??? no you cant do this by yourself at home. Jesus. Did you not see what the abscess looks like?

      • Parnasudha Karmodak Chakrabort

        By Untrained doctor I mean doctor having less knowledge but not me. Anyways I got a perfect doctor for my kitties and the operation is successful.

  • Bg Labelle

    Calcium Chloride is only available to a licensed veterinarian. I think it is important to specify this right away.